Hundred genes and many genomic regions have been identified as potentially causative for human neurodevelopmental disorders such as autism, intellectual disability/developmental delay, epilepsy, and schizophrenia (Cooper et al. 2011; Girirajan et al. 2011; O'roak et al. 2012; Fromer et al. 2014; Gilissen et al. 2014; Iossifov et al. 2014). While these studies are invaluable in providing a list of candidate genes, the molecular genetic basis of how disruption of these genes lead to disease is not completely understood (Chakravarti et al. 2013). Moreover, current strategies for functional evaluation of the discovered genes in animal models are limited due to a lack of highly sensitive and quantitative assays. Mouse models have been an impeccable resource for researchers, but functional genetics using mice are expensive, time consuming, and laborious. For decades, Drosophila melanogaster has proven to be a powerful model for genetic studies with about 75% of human disease genes having orthologs in flies (Reiter et al. 2001; Inlow and Restifo 2004). The genetic system and tools developed using Drosophila have provided us with a deeper understanding of cellular and molecular basis of several basic biological processes (St Johnston 2002). With the availability of such tools and high conservation of human disease-associated genes, the past decade has also seen the growth of Drosophila models to study human diseases (Wangler et al. 2015).
The Drosophila compound eye is a simple nervous system consisting of a symmetrical organization of approximately 750 ommatidia (Ready et al. 1976). Two-thirds of the vital genes in the Drosophila genome have been estimated to be required for eye development (Thaker and Kankel 1992). Although some genes are likely to be specific for eye development, other vital genes expressed in the eye are probably required for general cellular processes as well (Thomas and Wassarman 1999). Hence, phenotypic assessment of the eye can be extended to gene functions in other tissues. Since it is a dispensable organ for survival, studies using the fly eye have been used for understanding basic biological processes including cell proliferation and differentiation, neuronal connectivity, apoptosis, and tissue patterning (Karim et al. 1996). Classical studies on Drosophila have established a line of research using the fly eye as an experimental system for studying genetic effects (Ready et al. 1976; Meyerowitz and Kankel 1978; Moses and Rubin 1991; Thaker and Kankel 1992; Kumar 2012). For decades, the fly eye has been used as a system for functional screening of genes and genetic interactions involved in basic cellular processes, neurodevelopment and degeneration, and common complex diseases such as cancer and diabetes (Thomas and Wassarman 1999). We present a computational method for high throughput assessment of eye morphology in fruit flies. Flynotyper provides an accurate and automated method for eye phenotyping. Our algorithm is implemented as a software package called Flynotyper available for free download.
Eye imaging details for phenotyping using Flynotyper:
Eye imaging using bright field microscope: For light microscope imaging of adult eyes, we recommend immobilizing flies by freezing at -80°C and then mounting on blu-tack (Bostik Inc, Wauwatosa, WI). Please note that small errors in ommatidial detection can drastically change the interpretation of phenotypes, and Flynotyper will be more accurate when the fly eye is mounted in the right orientation. Mounting the samples at an angle can lead to incorrect phenotypic scores. We have used Olympus BX53 compound microscope with a LMPlanFL N 10X 0.25 NA air objective (Olympus, Tokyo, Japan), at 0.5X magnification and a z-step size of 12.1μm for imaging. We captured the images using CellSens Dimesion software (Olympus Optical) and then the image slices were stacked using Zerene Stacker (Zerene Systems, USA). Note that a higher quality contrast image would work best for ommatidial detection and obtaining phenotypic scores.
Eye imaging using Scanning Electron Microscope (SEM): Standard protocols for obtaining SEM images from flies can be followed. Flynotyper works best if the eyes are mounted in the right orientation. Manually cropping the region of interest in an SEM image gives a more accurate phenotypic assessment.
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